Skip to main content

Culture Of Animal Cells A Manual Of Basic Technique And Specialized Applications

Download Culture Of Animal Cells A Manual Of Basic Technique And Specialized Applications Full eBooks in PDF, EPUB, and kindle. Culture Of Animal Cells A Manual Of Basic Technique And Specialized Applications is one my favorite book and give us some inspiration, very enjoy to read. you could read this book anywhere anytime directly from your device. This site is like a library, Use search box in the widget to get ebook that you want.

Culture of Animal Cells A Manual of Basic Technique and Specialized Applications

Culture of Animal Cells   A Manual of Basic Technique and Specialized Applications Book
Author : R. Ian Freshney
Publisher : John Wiley & Sons
Release : 2016-01-05
ISBN : 9781118873656
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

This is the 7th edition of a textbook first published in 1983. It aims to provide basic instruction in the basic procedures of cell culture for newcomers to the field, including aseptic technique, safety and regulatory issues, equipment and materials, media preparation and sterilization, primary culture, propagated cell lines, characterization and authentication, contamination, cryopreservation, and quantitation. There are also a number of specialized protocols some of which have general interest, e.g. cell cloning, 3D culture, scale-up, STR profiling, and some with a with more limited readership, e.g. culture of some specialized cells. Some specialized protocols will be retained in the printed copy but others will be presented in electronic form only, depending on the anticipated readership. A number of minireviews, some by the author external review and some by invited authors will be added to give an overview of the applications of cell culture. New approaches and procedures have become available and new issues have arisen which require sections of the book to be updated. The increasing diversity of the applications of cell culture also need a revision of how certain topics are presented. The proliferation of specialized techniques requires that some of these now be presented online to avoid a further increase in size of the book. In addition the introduction of new topics requires that some of these be presented in mini-review form. Three reviewing editors have been appointed to advise on recent developments and trends and this will help to reshape the book in line with cutrrent demand. Some new features: There will be a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling (existing) and barcoding (new). Some specialized protocols, e.g. much of chapters 22, 23, and 27, will be removed and made available online (free to those who have purchased the print copy). This edition will focus more on more generally used techiniques and make other less used techniques available online. New mini-reviews will give insight into newer applications. More emphasis will be given to authentication and problems of misidentification. Illustrations will be updated as required.

Culture of Animal Cells

Culture of Animal Cells Book
Author : R. Ian Freshney
Publisher : Wiley-Liss
Release : 1993-12-29
ISBN : 9780471589662
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

This masterful third edition of Freshney's Culture of Animal Cells updates and considerably expands the scope of its predecessor and still enables both the novice and the experiences researcher to apply the basic and more sophisticated techniques of tissue culture. New Topics covered include: the use of molecular techniques in cell culture, such as DNA fingerprinting, fluorescence in situ hybridization, and chromosome painting cell interactions in cell culture new methods for separating cells new or refined methods for accessing cytotoxicity, viability, and mutagenicity experimental details for culture of specialized cells types not covered in previous editions new or refined techniques for visualizing clues, including time-lapse photography and confocal microscopy The revised and expanded third edition offers the following features: over 350 new reference to the primary literature an international list of cell banks an international listing of reagants and commercial supplies a subject index a glossary Also available: 0471169021 Culture of Animal Cells: A Multimedia Guide CD-ROM $150 est. From the reviews: "I strongly recommend this volume for any laboratory wishing to culture mammalian cells" - Biotechnology "It is not very often that it is possible to say of a book, 'I don't know how I managed without it previously.' Here is such a book" - Cell Biology International Reports

Freshney s Culture of Animal Cells

Freshney s Culture of Animal Cells Book
Author : R. Ian Freshney,Amanda Capes-Davis
Publisher : John Wiley & Sons
Release : 2021-02-17
ISBN : 1119513030
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

FRESHNEY’S CULTURE OF ANIMAL CELLS THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES Freshney’s Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more. The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text: Provides comprehensive single-volume coverage of basic skills and protocols, specialized techniques and applications, and new and emerging developments in the field Covers every essential area of animal cell culture, including lab design, disaster and contingency planning, safety, bioethics, media preparation, primary culture, mycoplasma and authentication testing, cell line characterization and cryopreservation, training, and troubleshooting Features a wealth of new content including protocols for gene delivery, iPSC generation and culture, and tumor spheroid formation Includes an updated and expanded companion website containing figures, artwork, and supplementary protocols to download and print The eighth edition of Freshney’s Culture of Animal Cells is an indispensable volume for anyone involved in the field, including undergraduate and graduate students, clinical and biopharmaceutical researchers, bioengineers, academic research scientists, and managers, technicians, and trainees working in cell biology, molecular biology, and genetics laboratories.

Animal Cell Culture

Animal Cell Culture Book
Author : John M. Davis
Publisher : John Wiley & Sons
Release : 2011-03-16
ISBN : 9780470975633
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.

Animal Cell Culture and Technology

Animal Cell Culture and Technology Book
Author : Michael Butler
Publisher : Garland Science
Release : 2003-12-25
ISBN : 1135325375
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Animal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for undergraduates, graduates and experienced researchers. Reflecting the latest developments and trends in the field, the new topics include the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation, and the humanization of antibodies for therapeutic use.

Animal Cell Culture

Animal Cell Culture Book
Author : R. Ian Freshney
Publisher : Oxford University Press
Release : 1992
ISBN : 0987650XXX
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Animal Cell Culture: A Practical Approach has sold over 10,000 copies since its publication in 1986, and remains one of the most popular titles in the series. This new edition takes account of the progress that has been made in the field. Although the basic principles remain the same,significant advances have been made in areas such as serum-free media, scale-up, and flow cytometry. As these techniques have developed as tools for the cell biologist, their availability to the non-specialist has also increased dramatically. Use of the tetrazolium salt MTT as a colorimetricindicator of viability has made a considerable impact on cytotoxity assay, and DNA fingerprinting has revolutionized the identification of individual cell strains. These, and other developments in the techniques described have made this new edition essential. The emphasis remains on presentingtechniques in a readily accessible form, with detailed protocols given throughout. This volume will be of use to researchers involved in both biological research and the commercial exploitation of animal cell culture.

Introduction to Cell and Tissue Culture

Introduction to Cell and Tissue Culture Book
Author : Jennie P. Mather,Penelope E. Roberts
Publisher : Springer Science & Business Media
Release : 2007-08-20
ISBN : 0585275718
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.

Cell Culture Technology

Cell Culture Technology Book
Author : Cornelia Kasper,Verena Charwat,Antonina Lavrentieva
Publisher : Springer
Release : 2018-10-10
ISBN : 3319748548
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

This textbook provides an overview on current cell culture techniques, conditions, and applications specifically focusing on human cell culture. This book is based on lectures, seminars and practical courses in stem cells, tissue engineering, regenerative medicine and 3D cell culture held at the University of Natural Resources and Life Sciences Vienna BOKU and the Gottfried Wilhelm Leibniz University Hannover, complemented by contributions from international experts, and therefore delivers in a compact and clear way important theoretical, as well as practical knowledge to advanced graduate students on cell culture techniques and the current status of research. The book is written for Master students and PhD candidates in biotechnology, tissue engineering and biomedicine working with mammalian, and specifically human cells. It will be of interest to doctoral colleges, Master- and PhD programs teaching courses in this area of research.

Culture of Human Stem Cells

Culture of Human Stem Cells Book
Author : R. Ian Freshney,Glyn N. Stacey,Jonathan M. Auerbach
Publisher : John Wiley & Sons
Release : 2007-07-16
ISBN : 9780470167519
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource. This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series—each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture. The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.

Cell Culture Bioprocess Engineering Second Edition

Cell Culture Bioprocess Engineering  Second Edition Book
Author : Wei-Shou Hu
Publisher : CRC Press
Release : 2020-03-06
ISBN : 1498762867
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

This book is the culmination of three decades of accumulated experience in teaching biotechnology professionals. It distills the fundamental principles and essential knowledge of cell culture processes from across many different disciplines and presents them in a series of easy-to-follow, comprehensive chapters. Practicality, including technological advances and best practices, is emphasized. This second edition consists of major updates to all relevant topics contained within this work. The previous edition has been successfully used in training courses on cell culture bioprocessing over the past seven years. The format of the book is well-suited to fast-paced learning, such as is found in the intensive short course, since the key take-home messages are prominently highlighted in panels. The book is also well-suited to act as a reference guide for experienced industrial practitioners of mammalian cell cultivation for the production of biologics.

Animal Cell Culture Techniques

Animal Cell Culture Techniques Book
Author : Martin Clynes
Publisher : Springer Science & Business Media
Release : 2012-12-06
ISBN : 3642804128
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.

Culture of Cells for Tissue Engineering

Culture of Cells for Tissue Engineering Book
Author : Gordana Vunjak-Novakovic,R. Ian Freshney
Publisher : John Wiley & Sons
Release : 2006-03-31
ISBN : 0471741809
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Step-by-step, practical guidance for the acquisition, manipulation,and use of cell sources for tissue engineering Tissue engineering is a multidisciplinary field incorporatingthe principles of biology, chemistry, engineering, and medicine tocreate biological substitutes of native tissues for scientificresearch or clinical use. Specific applications of this technologyinclude studies of tissue development and function, investigatingdrug response, and tissue repair and replacement. This area israpidly becoming one of the most promising treatment options forpatients suffering from tissue failure. Written by leading experts in the field, Culture of Cellsfor Tissue Engineering offers step-by-step, practicalguidance for the acquisition, manipulation, and use of cell sourcesfor tissue engineering. It offers a unique focus on tissueengineering methods for cell sourcing and utilization, combiningtheoretical overviews and detailed procedures. Features of the text include: Easy-to-use format with a two-part organization Logically organized—part one discusses cell sourcing,preparation, and characterization and the second part examinesspecific engineered tissues Each chapter covers: structural and functional properties oftissues, methodological principles, culture, cellselection/expansion, cell modifications, cell seeding, tissueculture, analytical assays, and a detailed description ofrepresentative studies End-of-chapter features include useful listings of sources forreagents, materials, and supplies, with the contact details of thesuppliers listed at the end of the book A section of elegant color plates to back up the figures in thechapters Culture of Cells for Tissue Engineering givesnovice and seasoned researchers in tissue engineering an invaluableresource. In addition, the text is suitable for professionals inrelated research, particularly in those areas where cell and tissueculture is a new or emerging tool.

Cell Culture and Its Application

Cell Culture and Its Application Book
Author : Ronald Acton
Publisher : Elsevier
Release : 2012-12-02
ISBN : 0323142613
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Cell Culture and Its Application covers the proceedings of the First International Cell Culture Congress Symposium, which focuses on how cell culture technology could impact on cell biology. The symposium aims to establish facilities for the cultivation of mammalian cells, which in turn would hopefully enhance basic cell biology research. The book is organized into four symposium and workshop sessions, encompassing 45 chapters. The opening chapter recognizes the interlocking relationship of cell culture technology and substantive cell biology. Chapters 2-5 describe the biochemical events that mark the cell cycle, with emphasis on occurrence of histone phosphorylation at each cycle. A discussion on cell differentiation, as a phenomena of interacting, inductive, and inhomogeneous cell populations, is included in these chapters. The second symposium session deals with signs of a revolution in progress in cell culture technology. This includes impact of tissue culture in physiological research course and in understanding of integrated physiology. The last two symposium sessions cover the large-scale production of virus from tissue cultures for cell antigens. An approach to the study of aging using diploid human cells in culture as a model system is also presented. It involves isolation and characterization of HLA antigens from cultured cells and their contribution to the study of disease. A brief discussion on mycoplasma contamination, microplasma-cell-virus interaction, and advantages and limitations of direct and indirect culture for primary isolation and detection of mycoplasma contamination is provided. The book then proceeds by discussing cell differentiation of specific cell or organ, such as testis, sensory cell, hepatocyte, embryonic muscle cell, and brain cortex. The concluding chapters cover nutritional requirements for cell growth, defined culture media for specific cell type, issues and problems related to large-scale cell production, and quality control. Cell biologists and researchers will find this book invaluable.

Basic Bioscience Laboratory Techniques

Basic Bioscience Laboratory Techniques Book
Author : Philip L.R. Bonner,Alan J. Hargreaves
Publisher : John Wiley & Sons
Release : 2011-08-24
ISBN : 1119956447
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

This unique, practical, pocket-sized guide and reference provides every first year bioscience student with all they need to know to prepare reagents correctly and perform fundamental laboratory techniques. It also helps them to analyse their data and present their findings, in addition to directing the reader, via a comprehensive list of references, to relevant further reading All of the core bioscience laboratory techniques are covered including: basic calculations and the preparation of solutions; aseptic techniques; microscopy techniques; cell fractionation ; spectrophotometry; chromatography of small and large molecules: electrophoresis of proteins and nucleic acids and data analysis. In addition the book includes clear, relevant diagrams and worked examples of calculations. In short, this is a 'must-have' for all first year bioscience students struggling to get to grips with this vitally important element of their course.

Fundamentals of Modern Bioprocessing

Fundamentals of Modern Bioprocessing Book
Author : Sarfaraz K. Niazi,Justin L. Brown
Publisher : CRC Press
Release : 2015-10-28
ISBN : 1466585749
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Biological drug and vaccine manufacturing has quickly become one of the highest-value fields of bioprocess engineering, and many bioprocess engineers are now finding job opportunities that have traditionally gone to chemical engineers. Fundamentals of Modern Bioprocessing addresses this growing demand. Written by experts well-established in the field, this book connects the principles and applications of bioprocessing engineering to healthcare product manufacturing and expands on areas of opportunity for qualified bioprocess engineers and students. The book is divided into two sections: the first half centers on the engineering fundamentals of bioprocessing; while the second half serves as a handbook offering advice and practical applications. Focused on the fundamental principles at the core of this discipline, this work outlines every facet of design, component selection, and regulatory concerns. It discusses the purpose of bioprocessing (to produce products suitable for human use), describes the manufacturing technologies related to bioprocessing, and explores the rapid expansion of bioprocess engineering applications relevant to health care product manufacturing. It also considers the future of bioprocessing—the use of disposable components (which is the fastest growing area in the field of bioprocessing) to replace traditional stainless steel. In addition, this text: Discusses the many types of genetically modified organisms Outlines laboratory techniques Includes the most recent developments Serves as a reference and contains an extensive bibliography Emphasizes biological manufacturing using recombinant processing, which begins with creating a genetically modified organism using recombinant techniques Fundamentals of Modern Bioprocessing outlines both the principles and applications of bioprocessing engineering related to healthcare product manufacturing. It lays out the basic concepts, definitions, methods and applications of bioprocessing. A single volume comprehensive reference developed to meet the needs of students with a bioprocessing background; it can also be used as a source for professionals in the field.

Studyguide for Culture of Animal Cells

Studyguide for Culture of Animal Cells Book
Author : Cram101 Textbook Reviews,Cram101 Textbook Reviews Staff
Publisher : Cram101
Release : 2013-05
ISBN : 9781478475804
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Never HIGHLIGHT a Book Again Includes all testable terms, concepts, persons, places, and events. Cram101 Just the FACTS101 studyguides gives all of the outlines, highlights, and quizzes for your textbook with optional online comprehensive practice tests. Only Cram101 is Textbook Specific. Accompanies: 9780872893795. This item is printed on demand.

Recombinant Glycoprotein Production

Recombinant Glycoprotein Production Book
Author : Virgínia Picanço-Castro,Kamilla Swiech
Publisher : Humana Press
Release : 2017-09-19
ISBN : 9781493973118
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

This volume covers a wide spectrum of techniques and approaches that are used in the upstream and downstream processing for recombinant glycoprotein production. Chapters guide the reader through state-of-art of therapeutic recombinant glycoproteins, explores the patent literature, expression systems used for glycoproteins production, methods employed in the downstream processing of different glycoproteins, and information about analytical tools and formulation strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Glycoprotein Production: Methods and Protocols aims to ensure successful results in the further study of this vital field

Principles of Fermentation Technology

Principles of Fermentation Technology Book
Author : Peter F. Stanbury,Allan Whitaker,Stephen J Hall
Publisher : Elsevier
Release : 2013-10-22
ISBN : 1483292916
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

This second edition has been thoroughly updated to include recent advances and developments in the field of fermentation technology, focusing on industrial applications. The book now covers new aspects such as recombinant DNA techniques in the improvement of industrial micro-organisms, as well as including comprehensive information on fermentation media, sterilization procedures, inocula, and fermenter design. Chapters on effluent treatment and fermentation economics are also incorporated. The text is supported by plenty of clear, informative diagrams. This book is of great interest to final year and post-graduate students of applied biology, biotechnology, microbiology, biochemical and chemical engineering.

Medicines from Animal Cell Culture

Medicines from Animal Cell Culture Book
Author : Glyn N. Stacey,John Davis
Publisher : John Wiley & Sons
Release : 2007-06-29
ISBN : 9780470723784
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Medicines from Animal Cell Culture focuses on the use of animal cell culture, which has been used to produce human and veterinary vaccines, interferon, monoclonal antibodies and genetically engineered products such as tPA and erythropoietin. It also addresses the recent dramatic expansion in cell-based therapies, including the use of live cells for tissue regeneration and the culture of stem cells. Medicines from Animal Cell Culture: Provides comprehensive descriptions of methods for cell culture and nutrition as well as the technologies for the preservation and characterisation of both the cells and the derived products Describes the preparation of stem cells and others for use in cell-based therapies – an area of burgeoning research Includes experimental examples to indicate expected results Covers regulatory issues from the UK, the EU and the USA and reviews how these are developing around the world Addresses the key issues of standardisation and validation with chapters on GLP and GMP for cell culture processes Delivering insight into the exciting world of biological medicines and directions for further investigation into specific topics, Medicines from Animal Cell Culture is an essential resource for researchers and technicians at all levels using cell culture within the pharmaceutical, biotechnology and biomedical industries. It is of value to laboratory managers in these industries and to all those interested in this topic alike.

Basic Science Methods for Clinical Researchers

Basic Science Methods for Clinical Researchers Book
Author : Morteza Jalali,Francesca Yvonne Louise Saldanha,Mehdi Jalali
Publisher : Academic Press
Release : 2017-03-31
ISBN : 012803078X
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)